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当前位置：农业与食品科学 > 2022年 2卷 (3)期

Open Access Article

Journal of Agriculture and Food Science. 2022; 2: (3) ; 1-9 ; DOI: 10.12208/j.jafs.20220022.

Preparation and sustained release of ascorbicacid nanoparticles based on γ-PGA
基于γ-PGA缓释抗坏血酸纳米颗粒的制备及缓释

作者： 温学康, 黄银凤, 余作龙 *

浙江树人学院生物与环境工程学院浙江杭州

*通讯作者：余作龙,单位：浙江树人学院生物与环境工程学院浙江杭州；

浙江省大学生科技创新活动计划（新苗人才计划）

发布时间: 2022-09-08 总浏览量: 447

PDF 全文下载引用本文万方数据（WANFANG DATA）

摘要

目的本研究是为探索一种能够保护抗坏血酸使其免受环境因素影响的载体和其缓释性能。方法本文以γ-聚谷氨酸(γ-PGA）和壳聚糖(CS)作为主要基材，向抗坏血酸和γ-PGA当中加入三聚磷酸钠（STPP）和聚乙烯吡咯烷酮(PVP)作为螯合剂和稳定剂，再将四种溶液混合之后滴加到CS当中，制备得到γ-PGA基抗坏血酸纳米颗粒。首先通过各原料不同浓度的配方对形成纳米颗粒的影响的单因素实验来确定单个最佳配方，然后对纳米颗粒进行粒径与电位分析和红外光谱分析，在粒径与电位分析中测定纳米颗粒的粒径（Z-AVE）、粒径分布指数（PDI）和表面电位（Zeta），通过比较分析各项结果来确定总体最佳配比，最后制得最佳配比的抗坏血酸纳米颗粒进行体外的缓释实验。结果本实验当中确定了γ-PGA基抗坏血酸纳米颗粒的最佳配比为：STPP浓度为0.4%、γ-PGA浓度为0.2%、PVP浓度为0.1%、CS浓度为0.02%。在最佳配比下，在pH1.2和pH6.8缓冲液中进行抗坏血酸的缓释实验，结果表明γ-PGA基抗坏血酸纳米颗粒在pH1.2和pH6.8溶液中，累积释放率分别达到了101.15%和100.80%。结论确定了γ-PGA基纳米颗粒缓释抗坏血酸的最佳组成配方，在两种模拟环境具有缓慢且持续释放的效果。

关键词： 抗坏血酸；γ-聚谷氨酸；纳米颗粒；制备；缓释

Abstract

Objective This study is to explore a carrier that can protect ascorbic acid from environmental factors and its sustained-release performance.
Methods In this paper γ- Polyglutamic acid（ γ- PGA) and chitosan (CS) were used as the main substrates, which were added to ascorbic acid and γ- Sodium tripolyphosphate (STPP) and polyvinylpyrrolidone (PVP) were added to PGA as chelating agents and stabilizers, and then the four solutions were mixed and added dropwise to CS to prepare γ- PGA based ascorbic acid nanoparticles. First, the single optimal formula was determined through the single factor experiment of the influence of the formulations of different concentrations of raw materials on the formation of nanoparticles, and then the particle size and potential analysis and infrared spectrum analysis were carried out on the nanoparticles. The particle size (z-ave), particle size distribution index (PDI) and surface potential (zeta) of the nanoparticles were measured in the particle size and potential analysis. The overall optimal ratio was determined by comparing and analyzing the results, Finally, the optimal ratio of ascorbic acid nanoparticles was prepared for in vitro sustained-release experiments.
Results In this experiment γ- The optimum ratio of PGA based ascorbic acid nanoparticles is: STPP concentration is 0.4% γ- The concentration of PGA was 0.2%, the concentration of PVP was 0.1%, and the concentration of CS was 0.02%. Under the optimal ratio, the sustained-release experiment of ascorbic acid was carried out in ph1.2 and ph6.8 buffers. The results showed that γ- The cumulative release rates of PGA based ascorbic acid nanoparticles in ph1.2 and ph6.8 solutions reached 101.15% and 100.80%, respectively.
Conclusion γ- PGA based nanoparticles are the best composition of slow-release ascorbic acid, which has the effect of slow and continuous release in two simulated environments.

Key words： Ascorbic Acid; γ-Polyglutamic Acid; Nanoparticles; Preparation; Sustained release

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引用本文

温学康, 黄银凤, 余作龙, 基于γ-PGA缓释抗坏血酸纳米颗粒的制备及缓释[J]. 农业与食品科学, 2022; 2: (3) : 1-9.

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