摘要
目的 检测华东地区鸡和猪源性弯曲菌的分布与分子分型特征，为华东地区弯曲菌的防控提供参考。方法 采集不同来源标本进行弯曲菌分离培养，采用纸片扩散法进行弯曲菌的药敏试验，采用PCR检测法进行分型检测。结果 共检测690份样品，其中87份弯曲菌阳性（12.17%）。所获菌株对头孢类、磺胺类抗生素高度耐药；分型检测共鉴定出50种ST型，其中ST-21（%）为主要型别。结论 华东地区弯曲菌分布和耐药状况呈现多样化特点，不同来源分离株耐药谱存在差异，且菌株ST型与耐药性间没有严格的对应关系。
关键词： 农产品；营销贸易；培训；实践；思考
Abstract
Objective To detect the distribution and molecular typing characteristics of Campylobacter spp. of chicken and pig origin in East China, and to provide reference for the prevention and control of Campylobacter spp. in East China. Methods Specimens from different sources were collected for isolation and culture of Campylobacter, drug sensitivity test of Campylobacter was performed by paper diffusion method, and typing detection was performed by PCR assay. Results A total of 690 samples were tested, of which 87 were positive for Campylobacter (12.17%). The obtained strains were highly resistant to cephalosporin and sulfonamide antibiotics; 50 ST types were identified in the typing test, among which ST-21 (%) was the main type. Conclusion The distribution and resistance status of Campylobacter in East China showed diversified characteristics, and there were differences in the resistance profiles of isolates from different sources, and there was no strict correspondence between ST types and drug resistance of strains.
Key words： agricultural products; marketing trade; train;practice; reflection
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