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当前位置：农业与食品科学 > 2024年 4卷 (2)期

Open Access Article

Journal of Agriculture and Food Science. 2024; 4: (2) ; 7-16 ; DOI: 10.12208/j.jafs.20240018.

Recombinase polymerase amplification technology and its application in food detection
重组酶聚合酶扩增技术及其在食品安全检测领域的应用

作者： 李龙¹, 王雨婷², 宫玉晶², 宋丽萍² *

1 北京市计量检测科学研究院北京

2 北京市食品检验研究院（北京市食品安全监控和风险评估中心）北京

*通讯作者：宋丽萍,单位：北京市食品检验研究院（北京市食品安全监控和风险评估中心）北京；

十四五国家重点研发计划（2022YFF1100902）

发布时间: 2024-06-22 总浏览量: 168

PDF 全文下载引用本文

摘要

随着人们对食品安全问题的日益重视，适合在现场开展食品安全快速检测的技术越来越受到中国学者的广泛关注。重组酶聚合酶扩增技术（Recombinase Polymerase Amplification, RPA）是近年来新建立的一种等温核酸扩增技术。与传统的核酸检测技术相比，RPA技术具有检测速度快，成本低，对设备依赖性小等特点，非常适合在条件简陋或资源不足的地方开展现场食品安全检测工作。本文介绍了RPA的扩增原理和最常见的RPA反应终点检测技术——侧向流层析技术（LED）的原理；综述了RPA技术在食源性致病微生物检测、物种成分鉴定、转基因食品鉴定以及食品中过敏原成分鉴定中应用；总结分析了RPA技术在食品安全检测领域应用的优势和面临的挑战；并展望了RPA技术的未来发展前景。

关键词： 重组酶聚合酶扩增技术（RPA）；等温核酸扩增技术；食品安全检测；食源性致病微生物；物种成分鉴定；转基因食品；过敏原成分

Abstract

As people pay more and more attention to food safety issues, the technology suitable for rapid food safety detection in the field has attracted more and more attention from Chinese scholars. Recombinase Polymerase Amplification (RPA) is a newly established isothermal nucleic acid amplification technique. Compared with the traditional nucleic acid detection technology, RPA technology has the characteristics of fast detection speed, low cost, little dependence on equipment, etc., which is very suitable for carrying out on-site food safety detection work in places with poor conditions or insufficient resources. This paper introduces the principle of RPA amplification and the principle of the most common RPA endpoint detection technology, lateral flow chromatography (LED). The application of RPA technology in detection of foodborne pathogenic microorganisms, identification of species components, identification of genetically modified food and identification of allergens in food was reviewed. The advantages and challenges of the application of RPA technology in the field of food safety inspection are summarized and analyzed. The future development prospect of RPA technology is also prospected.

Key words： Recombinase polymerase amplification technique (RPA); Isothermal nucleic acid amplification technique; Food safety testing; Foodborne pathogenic microorganisms; Species composition identification; Genetically modified food; Allergen component

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引用本文

李龙, 王雨婷, 宫玉晶, 宋丽萍, 重组酶聚合酶扩增技术及其在食品安全检测领域的应用[J]. 农业与食品科学, 2024; 4: (2) : 7-16.

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